congenic cd45 1 mice Search Results


female congenic b6 sjl ptprcapep3b boyj  (Inotiv)
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ly5.1 congenic b6 mice (cd45.1)  (Sankyo Labo Service KK)
90
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cd45 1 congenic mice b6 sjl  (Taconic Biosciences)
94
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congenic cd45 1 cd45 2 mice  (Charles River Laboratories)
86
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sjl ptprcapep3b boyj cd45 1 congenic mice  (Taconic Biosciences)
93
Taconic Biosciences sjl ptprcapep3b boyj cd45 1 congenic mice
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cd45 1 congenic marker line mice  (Jackson Laboratory)
86
Jackson Laboratory cd45 1 congenic marker line mice
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cd45 1 congenic c57bl 6 mice  (Shanghai Model Organisms Center)
86
Shanghai Model Organisms Center cd45 1 congenic c57bl 6 mice
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cd45.1 congenic c57bl/6 mice rbrc 00144  (BioResource International Inc)
90
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recipient wt b6 sjl ptprcapepcb boy  (Rad Source Technologies)
96
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wt c57bl/6j mice  (clea japan inc)
90
clea japan inc wt c57bl/6j mice
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type cbyj sjl b6 ptprca j  (Rad Source Technologies)
96
Rad Source Technologies type cbyj sjl b6 ptprca j
(A) Representative flow cytometry plots of bone marrow and spleen from arthritis afflicted RA-SKG and control SKG mice, gated on live <t>CD45</t> + Lin − cells and showing the CD11c + MHCII + DC cell population. Full gating hierarchy is presented in Figure S2A; average percentage of cells in DC gates for all mice in each group is indicated. (B) Absolute numbers of cDC1, cDC2a, cDC2b, and pDC cells in the bone marrow and spleen of arthritis afflicted RA-SKG and control SKG mice. DCs are defined as live CD45 + Lin − CD11c + MHCII + cells, where the lineage markers are CD3, CD19, NK1.1, Ly6G, F4/80, CD64 and TER119. DCs are subdivided into B220 + PDCA1 + pDCs and B220 − PDCA1 − cDCs, which are further subdivided into the cDC1, cDC2a, and cDC2b subsets based on the expression of XCR1, CD172a/SIRPα, and CLEC12A markers, as shown in Figure S2. Bars represent means ± SEM; data are from n=9 mice per group and consolidated from two independent experiments; statistical analyses used Student’s t -test; * p <0.05, ** p <0.01, ns – not significant. Marrow cell counts are per two tibias and femurs. (C-D) Expression of activation and checkpoint markers on DCs in the bone marrow and spleen of RA-SKG and control SKG mice, gating on DCs as live CD45 + Ly6G − F4/80 − CD11c + MHCII + cells. Bars represent means ± SEM; data from n=5-9 mice per group; MFI – mean or median fluorescence intensity; statistical analyses with Student’s t -test; * p <0.05, ** p <0.01, *** p <0.001; ns – not significant.
Type Cbyj Sjl B6 Ptprca J, supplied by Rad Source Technologies, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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c57bl 6  (Jackson Laboratory)
86
Jackson Laboratory c57bl 6
(A) Representative flow cytometry plots of bone marrow and spleen from arthritis afflicted RA-SKG and control SKG mice, gated on live <t>CD45</t> + Lin − cells and showing the CD11c + MHCII + DC cell population. Full gating hierarchy is presented in Figure S2A; average percentage of cells in DC gates for all mice in each group is indicated. (B) Absolute numbers of cDC1, cDC2a, cDC2b, and pDC cells in the bone marrow and spleen of arthritis afflicted RA-SKG and control SKG mice. DCs are defined as live CD45 + Lin − CD11c + MHCII + cells, where the lineage markers are CD3, CD19, NK1.1, Ly6G, F4/80, CD64 and TER119. DCs are subdivided into B220 + PDCA1 + pDCs and B220 − PDCA1 − cDCs, which are further subdivided into the cDC1, cDC2a, and cDC2b subsets based on the expression of XCR1, CD172a/SIRPα, and CLEC12A markers, as shown in Figure S2. Bars represent means ± SEM; data are from n=9 mice per group and consolidated from two independent experiments; statistical analyses used Student’s t -test; * p <0.05, ** p <0.01, ns – not significant. Marrow cell counts are per two tibias and femurs. (C-D) Expression of activation and checkpoint markers on DCs in the bone marrow and spleen of RA-SKG and control SKG mice, gating on DCs as live CD45 + Ly6G − F4/80 − CD11c + MHCII + cells. Bars represent means ± SEM; data from n=5-9 mice per group; MFI – mean or median fluorescence intensity; statistical analyses with Student’s t -test; * p <0.05, ** p <0.01, *** p <0.001; ns – not significant.
C57bl 6, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
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Image Search Results


(A) Representative flow cytometry plots of bone marrow and spleen from arthritis afflicted RA-SKG and control SKG mice, gated on live CD45 + Lin − cells and showing the CD11c + MHCII + DC cell population. Full gating hierarchy is presented in Figure S2A; average percentage of cells in DC gates for all mice in each group is indicated. (B) Absolute numbers of cDC1, cDC2a, cDC2b, and pDC cells in the bone marrow and spleen of arthritis afflicted RA-SKG and control SKG mice. DCs are defined as live CD45 + Lin − CD11c + MHCII + cells, where the lineage markers are CD3, CD19, NK1.1, Ly6G, F4/80, CD64 and TER119. DCs are subdivided into B220 + PDCA1 + pDCs and B220 − PDCA1 − cDCs, which are further subdivided into the cDC1, cDC2a, and cDC2b subsets based on the expression of XCR1, CD172a/SIRPα, and CLEC12A markers, as shown in Figure S2. Bars represent means ± SEM; data are from n=9 mice per group and consolidated from two independent experiments; statistical analyses used Student’s t -test; * p <0.05, ** p <0.01, ns – not significant. Marrow cell counts are per two tibias and femurs. (C-D) Expression of activation and checkpoint markers on DCs in the bone marrow and spleen of RA-SKG and control SKG mice, gating on DCs as live CD45 + Ly6G − F4/80 − CD11c + MHCII + cells. Bars represent means ± SEM; data from n=5-9 mice per group; MFI – mean or median fluorescence intensity; statistical analyses with Student’s t -test; * p <0.05, ** p <0.01, *** p <0.001; ns – not significant.

Journal: bioRxiv

Article Title: Trained Immunity Affecting Dendritic Cell Differentiation and Function in Rheumatoid Arthritis

doi: 10.1101/2025.02.28.640673

Figure Lengend Snippet: (A) Representative flow cytometry plots of bone marrow and spleen from arthritis afflicted RA-SKG and control SKG mice, gated on live CD45 + Lin − cells and showing the CD11c + MHCII + DC cell population. Full gating hierarchy is presented in Figure S2A; average percentage of cells in DC gates for all mice in each group is indicated. (B) Absolute numbers of cDC1, cDC2a, cDC2b, and pDC cells in the bone marrow and spleen of arthritis afflicted RA-SKG and control SKG mice. DCs are defined as live CD45 + Lin − CD11c + MHCII + cells, where the lineage markers are CD3, CD19, NK1.1, Ly6G, F4/80, CD64 and TER119. DCs are subdivided into B220 + PDCA1 + pDCs and B220 − PDCA1 − cDCs, which are further subdivided into the cDC1, cDC2a, and cDC2b subsets based on the expression of XCR1, CD172a/SIRPα, and CLEC12A markers, as shown in Figure S2. Bars represent means ± SEM; data are from n=9 mice per group and consolidated from two independent experiments; statistical analyses used Student’s t -test; * p <0.05, ** p <0.01, ns – not significant. Marrow cell counts are per two tibias and femurs. (C-D) Expression of activation and checkpoint markers on DCs in the bone marrow and spleen of RA-SKG and control SKG mice, gating on DCs as live CD45 + Ly6G − F4/80 − CD11c + MHCII + cells. Bars represent means ± SEM; data from n=5-9 mice per group; MFI – mean or median fluorescence intensity; statistical analyses with Student’s t -test; * p <0.05, ** p <0.01, *** p <0.001; ns – not significant.

Article Snippet: For competitive bone marrow transplantations, cohorts of wild type CByJ.SJL(B6)-Ptprca/J (JAX 006584, congenic for CD45.1) mice were irradiated with 2 doses of 4.5Gy, delivered 3 hours apart, in a RS-2000 irradiator (Rad Source), and engrafted with a 1:1 mix of bone marrow cells from CD45.2 + SKG mice with active arthritis (RA-SKG) and from control CD45.1 + allotype-marked mice, delivered as intravenous injection in sterile PBS.

Techniques: Flow Cytometry, Control, Expressing, Activation Assay, Fluorescence